A 3-D printable open source platform for fluorescence microscopy, optogenetics and accurate temperature control.
By A Maia Chagas, LL Prieto Godino, A Arrenberg and T Baden
Designed for flexibiliy and low-cost, the “FlyPi” is an optical microscope designed for behavioural work with fruit flies, zebrafish or C. elegans.
The system is based on a Raspberry Pi with camera, an Arduino microcontroller and a range of off-the-shelf electronic parts. A modular design provides for flexible control of a range of peripherals including options for optogenetic and thermogenetic stimulation and fluorescence microscopy. All mechanical parts are 3-D printed which includes a range of manual or motorised micropositioning options.
The basic setup can be put together for below 100€. The low price means that it can be implemented in under-funded labs across the world, for classroom teaching in schools as well as by home-enthusiasts who are not part of the traditional scientific establishment.
The MS, including complete build instructions and BOM is deposited on bioRxiv:
Chagas, A.M.$, Prieto Godino, L.L., Arrenberg, A. B. and Baden, T.$. The 100 Euro Lab: A 3-D Printable Open Source Platform For Fluorescence Microscopy, Optogenetics And Accurate Temperature Control During Behaviour Of Zebrafish, Drosophila And C. elegans. bioRviv doi: https://doi.org/10.1101/122812. direct link.
Other project materials
- Arduino, Python GUI and OpenSCAD code (GitHub)
- Project Summary (Hack-a-day)
- Remix: FlyPiScope (simplified model with camera integrated into base)